Bacterial methionine biosynthesis

Biosynthesis of methionine in liver.

Conversion of 1 carbon precursors to methionine methyl groups in a cell-free preparation was first demonstrated by Berg in this laboratory (2). Berg inactivated a pigeon liver extract by Dowex I-Cltreatment and dialysis and found that it could be activated by a boiled extract of pigeon liver. Methionine methyl formation in dialyzed liver estracts has been activated recently by mixtures of known...

Cystathionine y-Synthetase of SaZmoneZZa CATALYTIC PROPERTIES OF A NEW ENZYME IN BACTERIAL METHIONINE BIOSYNTHESIS

An enzyme catalyzing the synthesis of L-cystathionine from the succinyl ester of L-homoserine and L-cysteine was isolated from a Salmonella mutant blocked in another step of methionine synthesis, and derepressed for cystathionine y-synthetase formation. The approximate molecular weight of the enzyme was 155,000 and it was unchanged after removal of tightly bound pyridoxal phosphate. In the abse...

Cystathionine y-Synthetase of SaZmoneZZa CATALYTIC PROPERTIES OF A NEW ENZYME IN BACTERIAL METHIONINE BIOSYNTHESIS

An enzyme catalyzing the synthesis of L-cystathionine from the succinyl ester of L-homoserine and L-cysteine was isolated from a Salmonella mutant blocked in another step of methionine synthesis, and derepressed for cystathionine y-synthetase formation. The approximate molecular weight of the enzyme was 155,000 and it was unchanged after removal of tightly bound pyridoxal phosphate. In the abse...

Acetylhomoserine and methionine biosynthesis in Neurospora.

Role of methionine in bacterial chemotaxis.

The effect of methionine starvation on the motility and behavior of normal and nonchemotactic mutants of Salmonella typhimurium was investigated. Methionine starvation eliminates tumbling in the wild type, but fails to do so in an uncoordinated (frequent tumbling) mutant. In the mutant, methionine starvation significantly extends the period of smooth motility that follows a sharp temporal incre...